| 1. | Prof. Nathaniel Russell Middleton was born on 01 Apr 1810; died on 06 Sep 1890. Nathaniel married Annie Elizabeth DeWolf on 20 Sep 1842. Annie (daughter of Henry DeWolf and Annie Elizabeth Marsten) was born on 05 Feb 1815. [Group Sheet] [Family Chart]
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| 2. | Maria DeWolf Middleton |
| 3. | Annie E. Middleton |
| 4. | Alice Hopkins Middleton |
| 5. | Nathaniel Russell Middleton |
| 6. | Charlotte Helen Middleton Family/Spouse: Edward Padelford DeWolf. Edward (son of William Frederick DeWolf and Margaret Padelford Arnold) was born on 12 Jan 1848. [Group Sheet] [Family Chart] |